For research use only, not for use in diagnostic procedure.
Recombinant protein corresponding to human PPP1CB aa 100-327.
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
PPP1CB polyclonal antibody detects endogenous levels of PPP1CB protein.
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
PP-1B, PPP1CD, PPP1CB, MGC3672, PP 1B, PP-1B, PP1B, PP1B, PP1beta, PPP1CB, PPP1CD, Protein phosphatase 1 beta, Protein phosphatase 1 catalytic subunit beta isoform, Protein phosphatase 1 delta, Protein phosphatase 1, catalytic subunit, beta isozyme, Protein phosphatase 1, catalytic subunit, delta isoform, Serine threonine protein phosphatase PP1 beta catalytic subunit, Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody
Western blot analysis of PPP1CB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Mouse skeletal muscle tissue lysateLane 2: Rat brain tissue lysateLane 3: A431 cell lysateLane 4: SH-SY-5Y cell lysateLane 5: SiHa cell lysateLane 6: 293 cell lysateLane 7: Hela cell lysateLane 8: PC-3M cell lysateLane 9: HepG2 cell lysateLane 10: A549 cell lysate